---- Original message ----
>Date: Mon, 2 Jun 2008 17:04:38 +0100
>From: sajid akthar <
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>Subject: [ccp4bb] crystallisation
>To:
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>
>
>Dear All
>
>My protein size is ~30kD and crystallizes with
>19%Peg3350, 0.2M Nacl, and 0.1M Na Cacodylate buffer.
>
>Please refer the attached crystal image with this. The
>crystal looks like good enough for home source. These
>crystals appears in 4-5 days at room temp.
>
>Sometimes I'm getting crystals like this, but very few
>in 24 well tray. Most of the time, I found the drop
>contains needles. If I reduce the precipitant little
>bit, I wont find any change in the drop even after
>long time. Changing pH (or temp)of the buffer does not
>help me any better. The crystal appears only around
>5.5pH.
>
>The problem is mosaicity. This crystal diffracted in
>home source upto 3.2A and the mosaicity is 2.5degree.
>Almost all the good crystal like this having same
>mosaicity.
>
>Good cryo condition so far that I found was
>10%Glycerol with mother liquor. Other conditions
>weekens the diffraction quality or increase mosaicity.
>
>In many crystal I could see some crack in the middle
>of the crystal, it looks like twin crystal. Or the
>crystal appears with some sattelite crystals.
>
>Can anyone suggest me some good way to overcome these
>problems.
>
>Thankz
>
>Sajid
>
>
>
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