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Hi, Can anyone advise me what is currently the weakest protein-protein complex yet crystallised? Google searching turned up a paper from the Tromsø crystallography group (Helland et al. 1999, JMB 287, 923–942) in which a complex between beta-trypsin and a P1 mutant of BPTI with a Kd of 68 uM was described as belonging to the weakest complexes solved to date, but this article was from 1999 and much water has passed under the bridge since then. Thanks Derek _________________________________________________________________ Derek Logan tel: +46 46 222 1443 Associate professor fax: +46 46 222 4692 Molecular Biophysics mob: +46 76 8585 707 Centre for Molecular Protein Science Lund University, Box 124, 221 00 Lund, Sweden