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Thermolysin binds a catalytic zinc and several Ca ions for structural stability. See Holland et al. (1995) Protein Sci, 4, 1955. Hope that helps, Arthur Glasfeld Department of Chemistry Reed College 3203 SE Woodstock Blvd. Portland, OR 97202 USA On Jun 12, 2008, at 8:15 PM, Neeraj Kapoor wrote: > Hi all, > I recently came across a question about an interesting idea. > Does anyone know of an example of a protein binding to both Zn2+ > and Ca2+ at the same time? Are there any known well studied > precedents at all if any. Any help of insights would be very valuable. > > thanks, > Neeraj >