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We would like to use a particular value of anisotropy to constrain fibertracking. The parameter description to use this option from the command line is "-f". Would the minimum value of anisotropy follow this command or is there a more specific option to specifcy the value? Specifically, what would this look like on the command line? Thanks in advance for your help. Antonina Savostyanova GCAP, CBDB, NIMH > The explaination on http://www.fmrib.ox.ac.uk/fsl/fdt/ > fdt_probtrackx.html reads: > Use anisotropy to constrain tracking: Use this option if you want > the fractional anisotropic volumes (stored in merged_f<i>samples) > to influence the tractography. > The tracts stop if the anisotropy is lower than a random variable > between 0 (low anisotropy) and 1 (high anisotropy).