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Just a quick sanity check - you _are_ inverting the DTI->structural transform before using it to transform structural-space ROIs back to DTI space? Cheers. On 7 Apr 2008, at 03:03, Ravi Shetty wrote: > Hi I have been using FLIRT to register my T1 and DTI images to each > other but keep running into > problems. The registered images produced are always very close to > what they should be when I > overlay them onto the T1 image. The problem I have is the matrixes > don't always reflect the > registration image. I create a CC ROI mask on the T1 image/ > registered image and use the matrix I > produced to convert it back into difusion space. Now when I do this > with some subjects the ROI will > be spot on the CC and for others it will be off somewhere in the > cortex or even just into the black > void. I can't seem to find any rhyme or reason for this and the > variation of how off the matrix is does > not seem to be consistent, any help figuring this out would be > appreciated. Thanks. > --------------------------------------------------------------------------- Stephen M. Smith, Professor of Biomedical Engineering Associate Director, Oxford University FMRIB Centre FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK +44 (0) 1865 222726 (fax 222717) [log in to unmask] http://www.fmrib.ox.ac.uk/~steve ---------------------------------------------------------------------------