On Apr 14, 2008, at 2:10 PM, JOE CRYSTAL wrote:
Hi,
Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots available? Besides cost per tray and
maintenance cost, another important feature we consider is the time for
setting up a 96-well tray. It is an important factor since we are talking
about sub-microliter drops.
Best,
Joe
On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[log in to unmask]> wrote:
Al's Oil on the plates:
What a nightmare!!!!!!!
The oil creeps up the plate and over the sides. It dissolves adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.
Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
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-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: [log in to unmask]
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase the
proportion of protein in the - technical term - teeny drop to say two
thirds and (2) cover the drops with oil eg Al's oils
(silicone/paraffin). You still get vapor diffusion though the oil ,
and you'd like to slow up equilibration. of course (2) slows up the
robotics a little, but both should be trivial to set up..