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Depends on your robot, obviously. On Mon, Apr 14, 2008 at 10:32 PM, William Scott <[log in to unmask]> wrote: > Does this include the customary grieving period? > > > > On Apr 14, 2008, at 2:10 PM, JOE CRYSTAL wrote: > > Hi, > > > > > > Does anyone have information about how long it takes to set up a 96-well > > tray for the crystallization robots available? Besides cost per tray > > and > > maintenance cost, another important feature we consider is the time for > > setting up a 96-well tray. It is an important factor since we are > > talking > > about sub-microliter drops. > > > > > > Best, > > > > > > Joe > > > > On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[log in to unmask]> wrote: > > > > Al's Oil on the plates: > > > What a nightmare!!!!!!! > > > The oil creeps up the plate and over the sides. It dissolves > > > adhesives. > > > It makes me say bad words in multiple languages. > > > Bigger drops + no oil = fewer bad words. > > > > > > Lisa > > > -- > > > Lisa A. Nagy, Ph.D. > > > University of Alabama-Birmingham > > > [log in to unmask] > > > > > > -----Original Message----- > > > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of > > > Patrick Shaw Stewart > > > Sent: Friday, January 18, 2008 2:20 AM > > > To: [log in to unmask] > > > Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot > > > > > > One thing that people often overlook is that quite a lot of protein > > > can be lost by denaturation on the surface of the drop. This is more > > > significant for smaller drops. Two suggestions: (1) increase the > > > proportion of protein in the - technical term - teeny drop to say two > > > thirds and (2) cover the drops with oil eg Al's oils > > > (silicone/paraffin). You still get vapor diffusion though the oil , > > > and you'd like to slow up equilibration. of course (2) slows up the > > > robotics a little, but both should be trivial to set up.. > > > > > >