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Hi,


Does anyone have information about how long it takes to set up a 96-well tray for the crystallization robots available?  Besides cost per tray and maintenance cost, another important feature we consider is the time for setting up a 96-well tray.  It is an important factor since we are talking about sub-microliter drops.


Best,


Joe

On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[log in to unmask]> wrote:

Al's Oil on the plates:

What a nightmare!!!!!!!

The oil creeps up the plate and over the sides. It dissolves adhesives.

It makes me say bad words in multiple languages.

Bigger drops + no oil = fewer bad words.

Lisa

--

Lisa A. Nagy, Ph.D.

University of Alabama-Birmingham

[log in to unmask]

-----Original Message-----

From: CCP4 bulletin board [ mailto:[log in to unmask]] On Behalf Of

Patrick Shaw Stewart

Sent: Friday, January 18, 2008 2:20 AM

To: [log in to unmask]

Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

One thing that people often overlook is that quite a lot of protein

can be lost by denaturation on the surface of the drop.  This is more

significant for smaller drops.  Two suggestions: (1) increase the

proportion of protein in the - technical term - teeny drop to say two

thirds and (2) cover the drops with oil eg Al's oils

(silicone/paraffin).  You still get vapor diffusion though the oil ,

and you'd like to slow up equilibration.  of course (2) slows up the

robotics a little, but both should be trivial to set up..