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Off the top of my head: Phoenix Robot: ~2min (incl. reformatting) Hydra2plusOne: ~5min (incl. reformatting and washing of needles) Mosquito: ~2min (no reformatting, needles are disposable) Cartesian: ~20min (incl. washing) At 11:10 PM 4/14/2008, JOE CRYSTAL wrote: >Hi, > > >Does anyone have information about how long it takes to set up a >96-well tray for the crystallization robots available? Besides cost >per tray and maintenance cost, another important feature we consider >is the time for setting up a 96-well tray. It is an important >factor since we are talking about sub-microliter drops. > > >Best, > > >Joe > >On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy ><<mailto:[log in to unmask]>[log in to unmask]> wrote: >Al's Oil on the plates: >What a nightmare!!!!!!! >The oil creeps up the plate and over the sides. It dissolves adhesives. >It makes me say bad words in multiple languages. >Bigger drops + no oil = fewer bad words. > >Lisa >-- >Lisa A. Nagy, Ph.D. >University of Alabama-Birmingham ><mailto:[log in to unmask]>[log in to unmask] > >-----Original Message----- >From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of >Patrick Shaw Stewart >Sent: Friday, January 18, 2008 2:20 AM >To: <mailto:[log in to unmask]>[log in to unmask] >Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot > >One thing that people often overlook is that quite a lot of protein >can be lost by denaturation on the surface of the drop. This is more >significant for smaller drops. Two suggestions: (1) increase the >proportion of protein in the - technical term - teeny drop to say two >thirds and (2) cover the drops with oil eg Al's oils >(silicone/paraffin). You still get vapor diffusion though the oil , >and you'd like to slow up equilibration. of course (2) slows up the >robotics a little, but both should be trivial to set up.. > _______________________________________________ Jochen Muller-Dieckmann, Ph.D. Team Leader EMBL Hamburg Outstation c/o DESY, Notkestr. 85 D-22603 Hamburg Germany Ph: +49-40-89902-201 FAX: +49-40-89902-215 _______________________________________________ http://www.embl-hamburg.de/services/crystallisation