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Dear Peter I would like to add my voice to yours, I am also worried about our HDL method and the increasing number of subjects reported with low HDL cholesterol using Beckman technology, further to that I have contacted the company, they are looking into that, they admitted to problem with some lot number and correction factor, I am still in the process of investigating that. Looking forward from input from other departments. We have brought up this issue to our Wessex ACB group. Regards Ali Dr Ali Al-bahrani MBCh.B MSc. CSci. FRCPath. Consultant Chemical Pathologist and HOD of Chemical Pathology St Mary's Hospital Newport Isle of Wight PO30 5TG United Kingdom Te: 01983 534859/534917 Fax. 01983 825437 -----Original Message----- From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Sharpe, Peter Sent: 22 April 2008 09:47 To: [log in to unmask] Subject: HDL-C Dear Colleagues, I have great concerns regarding measurement of HDL-C in samples with raised triglycerides. The recent NEQAS for Lipid Investigations report (Distribution 84) demonstrated marked differences between methods. Specimen 84B had triglycerides approx 5 mmol/l; we use the Roche generation 3 HDL-C method and obtained an HDL-C result of 0.94 mmol/l. The ALTM value was 1.18 mmol/l and I note that some labs were getting results > 1.5 mmol/l. In specimen 84C, trigs were approx 7.1 mmol/l. We obtained an HDL-C of 0.68 mmol/l, the ALTM value was 0.96 mmol/l and many labs obtained HDL-Cs over 1.1 mmol/l. The question is which method is obtaining the correct result for the HDL-C in the presence of raised trigs. The Roche generation 3 method is markedly negatively biased compared to the others. Don't forget we are using the HDL-C to calculate the 10 year risk of Cardio-Vascular disease and with the profound method-dependency of the HDL-C result, should we be continuing to do so? We could be either seriously under- or over-estimating the risk. Furthermore, since we moved to Roche generation 3 HDL-C, we are now getting up to 10 profoundly low HDL-C concentrations (< 0.6 mmol/l) out of the 600 lipid profiles we analyse each day. Can I believe these results? Any thoughts or comments would be welcomed. Best wishes Peter Dr Peter C Sharpe Consultant Chemical Pathologist Southern Health & Social Care Trust Tel 028 38612657 Fax 028 38334582 This email is confidential and intended solely for the use of the individual(s) to whom it is addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of Southern Health and Social Care Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please notify the sender. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/