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杨柳青 wrote: > Hello,everyone! > I have a question,is the expression vector effect crystallization much? > For example,"PQE2-protein" structure has 2 molecules in one > unit-cell,"PQE70-protein single mutant" has 5molecules in one > unit-cell.Is the vector effect the results or the mutant itself? > Can protein with some kind vector does not produce crystals? > Thank you very much! > Best wishes! > liuqing > > ------------------------------------------------------------------------ > 使用新一代 Windows Live Messenger 轻松交流和共享！ 立即体验！ > <http://messenger.live.cn/> The expression vector is essentially irrelevant to protein crystallization, as it is only the means by which the recombinant protein is produced. However, the protein structure does have everything to do with crystallization conditions and results. Single amino acid mutations, or the presence of purification tags, can have profound effects on the unit cell symmetry and size of protein crystals. In addition, a single protein can, and often does, crystallize under different crystallization conditions in wildly different space groups and asymmetric units. -- ------------------------------------------------------------------------ Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [log in to unmask]