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 Hi,
 everyone, who has much more experience about the 2-D crystallization of membrane proteins using the dialysis method.

1)   how to avoid the change of whole sample's volume  after dialysis?
2)   can you tell me some tricks during making grids, that is, when we put dialysis sample on grids, your experiences?

Thanks in advance, Thanks your kindness;

best regards,

gxzhao

lab of electron cryo-microscopy
school of biology
Georgia institute of technology
Atlanta, GA 30318