Dear All,
I am working with a Bifunctional protein of molecular weight ~60 kDa.
I have a 3.3 angstrom native dataset. The matthews number show there are 6 molecules in the asymmetric unit.
The
structures of the individual domains are already known from
prokaryotes. The sequence identity with the known structures are about
30%.
I have tried molecular replacement using the two parts as models
respectively with CNS, MOLREP, PHASER etc. However I always get the
solution for one domain. I have also tried to fix that domain and find
the other one. But none of the programs can find a solution.
I am trying to model build the correct sequence of one domain using a
density modified (using CNS), NCS averaged (using RAVE) map but the map
does not look very good. The side chains are not clear. That might be
due to the fact that I am only having a partial model.
Any suggestion will be appreciated.
Thanks.
Ms. Anjali Mehta
