 |
 |
Dear protein purifiers, Off topic question: Is there a general rule for how fast you can load, wash and elute from affinity columns, e.g. glutathione agarose? The product insert from Sigma says load under gravity flow. For the volume of cell lysate I have, gravity loading would take an excruciatingly long time. I want to hook up a peristaltic pump to speed things along, but don't really have a feel for just how fast one can load a column in general (I realize this is also dependent on the construct, the buffer, etc). What about the subsequently wash or elution? Thanks for help Eric -- D. Eric Dollins, Ph.D. C266 LSRC, Research Dr. Duke University Medical Center Durham, NC 27710 (919) 681-1668, [log in to unmask]