Dear Jeff:<br>The sequence here is NYT. I think it is glycosylated here, isn&#39;t it?<br>Thanks a lot. <br><br><div class="gmail_quote">On Jan 8, 2008 11:37 AM, Jeff Lee &lt;<a href="mailto:[log in to unmask]"><a href="/cgi-bin/wa-jisc.exe?LOGON=A3%3Dind0801%26L%3DCCP4BB%26E%3D7bit%26P%3D582609%26B%3D------%253D_Part_40256_18414154.1199763903845%26T%3Dtext%252Fhtml%3B%2520charset%3DISO-8859-1%26pending%3D" target="_parent" >[log in to unmask]</a>
</a>&gt; wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;"><div style="">
Hi,<div><br></div><div>Do you know what is the protein sequence in this region. &nbsp;If it is glycosylated, it should have a distinctive motif (N-X-S/T). &nbsp;You might have the first NAG attached to the Asn if the motif is there.
</div><div><br></div><div>Jeff</div><div><br></div><div>&nbsp;<br><div><div><div></div><div class="Wj3C7c"><div>On Jan 7, 2008, at 7:19 PM, Jiamu Du wrote:</div><br></div></div><blockquote type="cite"><div><div></div><div class="Wj3C7c">
Dear All:<br>I am refining a structure at 2.6 A reslution. The protein is expressed in CHO cell, so it might be <font size="-1">glycosylated.<br>While refining an Asn residue as shown in the figure of the attachment, I found some strange density extended beyond the side chain of Asn. The sigma level for the fofc map of the figure in the attachment is as high as 
3.0. My protein buffer is Tris and NaCl, and the reservior is PEG and NH4Cl.</font><font size="-1"> I believe this is not water mlecules. Considering the residue is Asn, I guess it might be carbohydrate. <br>Is this density like a carbohydrate molecule? How to model this density? 
<br>Thanks and Happy New Year.<br clear="all"></font><br>-- <br>Jiamu Du<br>State Key Laboratory of Molecular Biology<br>Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences<br></div></div>
Chinese Academy of Sciences (CAS)<span>&lt;asn.JPG&gt;</span></blockquote></div><br></div></div></blockquote></div><br><br clear="all"><br>-- <br>Jiamu Du<br>State Key Laboratory of Molecular Biology<br>Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences
<br>Chinese Academy of Sciences (CAS)