Dear all

 

Last year we performed a UFC extraction where after adding the urine sample to the dichloromethane and vortexing, the mixture became extremely viscous and after 7 minutes on a multi-vortexer, practically solid. The same thing has now happened on a second urine from this patient and even stranger, to a different patient’s sample on the same run.  Has anyone had similar experiences or even know of an explanation? I’ve trawled pubmed (and google) to no avail.

 

For background, the first patient has generalised proteinuria (P/Crn ratio >900 mg/mmol on sample in dec, multistix +++ on UFC sample) and glycosuria (++),  the second patient has + protein and –ve glucose. Our extraction uses 3mL cold dichloromethane & 600 uL borate-preserved urine.

 

Gareth

 

 

Gareth Llewelyn ap Huw Jones
Senior Clinical Biochemist

Dept. Clinical Biochemistry
Royal Free Hospital
London NW3 2QG

 

Tel: 020 7794 0500 ext 38856
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