Dear all
Last year we performed a UFC extraction where after adding the urine
sample to the dichloromethane and vortexing, the mixture became
extremely viscous and after 7 minutes on a multi-vortexer, practically
solid. The same thing has now happened on a second urine from this
patient and even stranger, to a different patient's sample on the same
run. Has anyone had similar experiences or even know of an explanation?
I've trawled pubmed (and google) to no avail.
For background, the first patient has generalised proteinuria (P/Crn
ratio >900 mg/mmol on sample in dec, multistix +++ on UFC sample) and
glycosuria (++), the second patient has + protein and -ve glucose. Our
extraction uses 3mL cold dichloromethane & 600 uL borate-preserved
urine.
Gareth
Gareth Llewelyn ap Huw Jones
Senior Clinical Biochemist
Dept. Clinical Biochemistry
Royal Free Hospital
London NW3 2QG
Tel: 020 7794 0500 ext 38856
Email: [log in to unmask]
or [log in to unmask]
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