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Covalent modification?? Bias in 2fo-fc map? Can you show us a pic of offending density? Dave On 28/12/2007, Brenda Patterson <[log in to unmask]> wrote: > > Hello, > > I am fairly new to this lark so please forgive me if this question is > unclear, > but it is really puzzling me. > > I have used phaser to generate a molecular replacement structure of my > target > (which has 100% identity to my template) and this particular crystal I had > soaked with a ligand. > > I have a density in my difference map which resembles my ligand. The > thing is, > it overlaps the density map of my structure and if I were to place the > ligand > in that map, then there would be a steric clash. Obviously I would expect > the > ligand to be near to the structure, but not overlapping it? I am > uncertain how > to proceed? > > Any helpful suggestions please? > > > Thanks in advance > > Brenda > -- ============================ David C. Briggs PhD Father & Crystallographer http://www.dbriggs.talktalk.net AIM ID: dbassophile ============================