Dear all,

When running Pointless 1.2.0 (see summary below), I get the cubic space group P 4 3 2 

(mosflm gave P 2 3 or P 4 3 2) 

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Best Solution     space group P 4 3 2

   Reindex operator: [h,k,l]

   Laue group probability:             1.000

   Systematic absence probability:     0.975

   Total probability:                  0.975

   Space group confidence:             0.964

   Laue group confidence               1.000

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However, although I have multiple models (ensemble) with approximately 30% sequence identity (44% homology), I'm not able to get any reasonable solution with Phaser.

As we know the protein can form dimers and even trimers, is it possible that Poinless is giving a higher space group because of pseudo-symmetry in this case..?

Does anyone has got experience with Pointless and this kind of space group please..?

Many thanks

Kristof

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Kristof Van Hecke, PhD

Biomoleculaire Architectuur

Celestijnenlaan 200 F

B-3001 Heverlee (Leuven)

Tel: +32(0)16327477

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