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Dear Saemann, Thanks for your reply. I was wondering if the two normalize buttons are doing the same job, how come I get different results when I apply them on the same subject using the same template?? I get much better registration (in terms of normalized cross correlation values) in SPM5 than in SPM2 registering to ICBM152. Thanks. Regards, Amir Saemann Philipp wrote: > Hello, > > (1) The Colin27 is the brain of a single subject, and cannot provide > probability maps as needed by SPM5 for segmentation. > So unless there is a very specific reason to no use the default priors in > the tmp folder, these default option would be the first solution. > > (2) No. Unified segmentation runs via the segmentation button and works > with three tissue priors. The normalize button - to my knowledge - is about > doing the same as compared with normalization in SPM2. In both versions you > can either estimate the deformation matrix or estimate and write out the > normalized brains. > > Hope this helps - if not, best explain what the goal of the SPM2/SPM5 > comparison is, > there have been mails on the differences between these two versions, > > Philipp > > Max Planck Institute of Psychiatry > NMR Research Group > Kraepelinstr. 2-10 > 80804 Munich > Mail: [log in to unmask] > Phone: 0049-89-30622-413 > > -- ________________________________ Amir M. Tahmasebi Ph.D. Student, Medical Image Analysis Lab. School of Computing, Queen's University, Kingston, Ontario CANADA K7L 3N6 ________________________________ Tel: (613) 533-2797 Web: www.cs.queensu.ca/~tahmaseb