There are some screens for sale based on experiences of large Structural
Genomics consortia, the JCSG (+) screen is an example. Screens like these
are often biased towards the type of organism targeted by the consortium
(e.g. eukaryotic/prokaryotic)
Flip
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
William Scott
Sent: Tuesday, October 30, 2007 8:09 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] Crystal Screens
Dear Ray:
The Crystal Screen I is based on a screen that was developed over a period
of years in Sung-Hou Kim's lab, and is heavily weighted with conditions that
were successful for obtaining the protein crystals that were particular to
his lab at that time (which was when I was a graduate student and before, so
Ronald Reagan was still President and my rent in Berkeley was $250/mo). The
Natrix screen is based on one I made for screening RNA crystals using
primarily PEGs in 1993, with no particular insight.
The screens are convenient, but they aren't magical. Don't be shy about
making your own up.
Bill Scott
On Tue, 30 Oct 2007 14:22:40 -0400
Ray Changrui Lu <[log in to unmask]> wrote:
Hello all,
I am forwarding this email as requested by a lab member due to
technical difficulties in school mail accounts.
Ray
>Hello,
>
>I usually try out Hampton crystal screen I & II, and PEG/ion screens
>on my proteins, sometimes varying protein concentrations and
>incubation temperature. If no hints are obtained, I will go back to
>test alternative constructs and purification protocols. With more
>and more companies selling crystal screens, and the accumulation of
>knowledge from structural genomics projects, I worry that my
>practice might have been outdated. I wonder if people could share
>with me:
>
>1) What is your favorite set of crystal screens that maximize the
>chance of getting the crystals while minimize the sample usage, and
>perhaps more importantly, when do you decide to stop for better
>constructs?
>
>2) Are there any other screens targeting nucleic acid and NA-protein
>complexes other than the Natrix and the Nucleic Acid Mini Screen
>sold by Hampton Research? Here I mean real different conditions, not
>just a re-label.
>
>I'd appreciate your thoughts.
>
>Best
>
