Tim, See Julian Barth's book - Biochemical Investigations in Laboratory Medicine (ACB Venture Publications). States: 09.00: Start 24 hr urine, administer 500 mg d-penicillamine; 21.00 administer second 500 mg dose; 09.00 complete 24 hr collection. Also states a combination of abnormal results is probably necessary for diagnosis - thresholds: Caeruloplasmin < 0.2 g/L; Se Copper <12 umol/L; Urine Copper > 4umol/24 hr; Ur copoer post penicillamine >25 umol/24hr." Joe Dr JP Begley, Clinical Biochemistry Department, Poole & Royal Bournemouth Hospitals, Dorset ________________________________ From: Clinical biochemistry discussion list on behalf of Reynolds Tim Sent: Thu 18/10/2007 09:59 To: [log in to unmask] Subject: Penicillamine challenge in Wilson's disease I need to organise a penicillamine challenge test for a patient with probable Wilson's disease there are lots of references to this test on the net but they all give instructions like this: Following penicillamine 0.5 g 12 hourly x 2, urine copper exceeds 25 micromol/24 hour in 88% patients with WD and 2% with other liver disorders. Does anyone know the precise time schedule relating the penicillamine should be taken and the time the urine collection starts? i.e. is it start both penicillamine and collection at the same time, or is there a lag between taking the drug and starting th eurine collection? TIM ************************************************************************ ************* Prof. Tim Reynolds, Queen's Hospital, Belvedere Rd, Burton-on-Trent, Staffordshire, DE13 0RB work tel: 01283 511511 ext. 4035 work fax: 01283 593064 work email: [log in to unmask] home email: [log in to unmask] ************************************************************************ ************** IMPORTANT: This email is intended for the use of the individual addressee(s)named above and may contain information that is confidential privileged or unsuitable for overly sensitive persons with low self-esteem, no sense of humour or irrational religious beliefs [if you want to believe in fairy stories and hug pixies that's up to you]. If you are not the intended recipient, any dissemination, distribution or copying of this email is not authorized (either explicitly or implicitly) and constitutes an irritating social faux pas. Unless the word absquatulation has been used in its correct context somewhere other than in this warning, it does not have any legal or grammatical use and may be ignored. No animals were harmed in the transmission of this email, though the kelpie next door is living on borrowed time, let me tell you. Those of you with an overwhelming fear of the unknown will be gratified to learn there is no hidden message revealed by reading this backwards, so just ignore that Alert Notice from Macroshaft. However, by pouring a complete circle of salt around yourself and your computer you can ensure that no harm befalls you and your pets. If you have received this eMail in error, please add some nutmeg and egg whites, whisk, and place in a warm oven for 40 minutes. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk <http://www.acb.org.uk/> List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/