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Have you considered it to be a disordered terminus with some waters extending the density (at least in B1 from your figures)? Anthony ________________________________ Anthony Addlagatta, PhD Institute of Molecular Biology University of Oregon Eugene, OR-97403 Phone: (541) 346-5867 Fax: (541)346-5870 Web: http://uoregon.edu/~anthony On Sep 28, 2007, at 1:01 PM, Jie Liu wrote: > Sure, here they are. > > There are three copies in ASU, all have more or less similar > density, blue contour at 1.0sigma for 2fo-fc and magenta > at 2.5sigma for fo-fc. The shown residues are the second > valines which fit well. > > Thanks. > > Jie > > [log in to unmask] wrote: > >> Can you show the map? >> >> Anthony >> >>> Dear all >>> >>> I am refining a peptide structure with an N-terminal throenine. >>> However, the density map of the N-terminal part looks strange, >>> the first throenine does not fit in well while the second valine >>> fits very well. It seems that this throenine was modified somehow >>> during protein production. The peptide was expressed in E.Coli >>> and cleavaged by Formic Acid and Cyanogen Bromide, then >>> purified by HPLC and lyophilized. >>> >>> Does anyone have similar experience of a modified N-terminal >>> throenine? If so, please kindly point to me a reference. >>> >>> Thank you. >>> >>> Jie Liu >>> >>> <A1.jpg> >>> <B1.jpg> >>> <C1.jpg>