Hi,
They’re likely both BME adducts,
just in the first case the CH2CH2OH portion is way more disordered.
Artem
From: CCP4 bulletin
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Sent: Monday, August 13, 2007 9:59
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Subject: [ccp4bb] extra density on
Cysteine
Dear all,
I
am refining a 2.0A structure. I found that there were some extra density on two
cysteines, even though I have added 5mM BME in the protein buffer.
I
am wondering whether the first one (Cys292) is a bme and the second one is an
oxidized cysteine. Any suggestion?
I
attached the images for your reference. thanks
Regards
_________________________________________
Xu Ting ,Ph.D
Fax: +65 6722 2916
Phone: +65 6722 2980