Dear All.

 

Is there any disadvantage of using the anomalous data set to do refinement?

 

I recently solved a structure using Se-SAD.  The anomalous data set was used in refinement with CNS. It gives decent result. Because traditionaly, after phasing we will use the nonanomalous data set in refinement. I am just curious if anybody has tried using the anomalous one and what's the difference between using anomalous and nonanomalous data in refinement..

 

 

Thank You