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Hi, They're likely both BME adducts, just in the first case the CH2CH2OH portion is way more disordered. Artem _____ From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of [log in to unmask] Sent: Monday, August 13, 2007 9:59 PM To: [log in to unmask] Subject: [ccp4bb] extra density on Cysteine Dear all, I am refining a 2.0A structure. I found that there were some extra density on two cysteines, even though I have added 5mM BME in the protein buffer. I am wondering whether the first one (Cys292) is a bme and the second one is an oxidized cysteine. Any suggestion? I attached the images for your reference. thanks Regards _________________________________________ Xu Ting ,Ph.D 10 Biopolis Road Singapore 138670 Fax: +65 6722 2916 Phone: +65 6722 2980