There might be many reasons.. Do you have a spacegroup with alternate indexing? If so the two crystals may not be using the same convention. See $CHTML/reindexing.html for more details. Or is one data set a high resolution pass and one a low resolution pass? Sometimes then there are saturated spots which mess up the scaling.. I usually merge both sets independently first - cad them together and run SCALEIT to check the degree of agreement. SCALEIT gives a useful list of outliers and you might be able to pinpoint the problem from that. Kianoush Sadre-Bazzaz wrote: > Dear CCP4, > > I have a couple of datasets native and heavy-atom-derivatized. The > spacegroup and cell dimensions of all are the same (or very > similar). For some reason I cannot merge any of the datasets > together. If for example I rescale one native dataset by itself, my > Rmerge is about 10%, but when I add 50 images (or so) of another, > supposedly isomorphous crystal, my Rmerge goes up to 20%. > > I was wondering if anyone has encountered a similar problem or can > offer any suggestions. Thanks so much, > Kianoush > > Choose the right car based on your needs. Check out Yahoo! Autos new > Car Finder tool. > <http://us.rd.yahoo.com/evt=48518/*http://autos.yahoo.com/carfinder/;_ylc=X3oDMTE3NWsyMDd2BF9TAzk3MTA3MDc2BHNlYwNtYWlsdGFncwRzbGsDY2FyLWZpbmRlcg--%20>