----- Original Message -----
Sent: Thursday, August 30, 2007 12:01
PM
Subject: 3 centrifugation question
1. This may sound simplistic, but I haven't been able to find
documentation. I think the relative centrifugal force (RCF) times the
time of centrifugation can be used to calculate equivalent degrees of
centrifugation.
For example, centrifuging at 1500 g for 10 min is equivalent to
centrifuging at 5000 g for 3 min. Right?
2. Centrifuging whole blood to obtain the serum or plasma at too high of
a RCF might damage cells during the process. Besides the possibility of
some hemolysis leading to artifactual increases in K+, LD, etc., are there any
other chemistry analytes that might be affected?
3. I always thought satisfactory centrifugation could be accomplished
more quickly in fixed angle rotors than in swinging bucket rotors.
However, BD stipulates a longer centrifugation time for fixed angle than for
swinging bucket. (This might only apply to gel separator tubes.) Did I
always think wrong (on this issue, not to mention many more).
Thanks. -Jim
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