Hi, In my experience Analysis doesn't "automatically" update the ppm range for z and z2 dimensions of a 4D experiment if you map your dimensions to different axes. I have to "kickstart" Analysis by making adjustments to the Minimum and/or Maximum aliased frequency in the Edit spectrum referencing popup (Experiments, Edit Spectrum, Reference). It doesn't seem to matter so much exactly what you do, but you have to make some adjustment to the aliased frequency. I've attached a before and after screenshot to give you a better idea what I mean. Also see discussion at http://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0612&L=CCPNMR&P=R44284&I=-3 and http://spot.colorado.edu/~jdouglas/ccpn/cantseexaplane.html (the images are tiff files; some browsers might not display, but you can right-click and download them separately) *** One final comment. I would *strongly* recommend that you transpose you matrix externally, NOT in analysis. What I mean is that it is much better to use nmrPipe to transpose your matrices to get the appropriate dimensions in the xy plane. With 4Ds it seems that Analysis really gets bogged down trying to display contours and navigate through 4Ds with different dimension mapping. I use a very fast new intel mac with gobs of RAM, etc. and I find that analysis is almost unusable. Even trying to make the screenshots, analysis got stuck for several minutes and crashed when I was trying to get to the appropriate plane in window2. Hope that helps, Justin On 7/21/07, Jo Claridge <[log in to unmask]> wrote: > > Hi > > I have a 4D 13C 15N edited NOESY experiment which I want to assign in > Analysis. I can load the data but I can't get a view with the axes: x=H > y=C z=HN z2=N. I keep getting two identical HN axes at x and z. I'm sure > this is my fault but I can't find a way round it. Would I have to create a > new experiment with the proton dimensions defined diferently? > > cheers, > Jo Claridge >