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Hi FSL, I'm trying to use melodic to denoise my fMRI dataset. I'm using SPM5 for preprocessing as well. The issue I'm having is when I try to move my 4D dataset (in which I have removed certain unwanted components) back to 3D format with avwsplit, SPM5 can no longer display this data. Here's what I'm doing exactly. I realign with SPM5 (so some affine transforms will be saved in the header) I convert the 3D data to 4D using avwmerge I run melodic on the data and remove unwanted components I convert the 4D data back to 3D using avwsplit anc hopefully continue with preprocessing. BTW, it seems that if one just uses avwmerge to move data from 3D to 4D and then back from 4D to 3D, there isn't an error. However, it seems some of the affine transformation parameters might be altered. The real problem is after using melodic - the dimensions, the voxel size, the data type, the origin, as well as the transformation matrix are all changed. please help! Kind Regards, Josh Bueller