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> I'm having the same problem, except that with me analysis crashes > immediately when using the Ax/(1+Ax) function - also with a segmentation > fault. Could that be an uncaught division by 0 somewhere? ;) Quite possibly (although not in the Python world, given the errors). As in my prev post use the other fit (if appropriate) in the mean time: It is better all round and has a more interpretable meaning for binding. T. ------------------------------------------------------------------------------- Dr Tim Stevens Email: [log in to unmask] Department of Biochemistry [log in to unmask] University of Cambridge Phone: +44 1223 766018 (office) 80 Tennis Court Road +44 7816 338275 (mobile) Old Addenbrooke's Site +44 1223 364613 (home) Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23 United Kingdom http://www.pantonia.co.uk ------------------------------------------------------------------------------- ------ +NH3CH(CH(CH3)OH)C(O)NHCH(CH(CH3)CH2CH3)C(O)NHCH(CH2CH2SCH3)CO2- ------- -------------------------------------------------------------------------------