What is the best value of it for different resolution
data?
I don't know exactly, but I like to use the minimum (0.01)
for "bad" data (that is high Rmerge, high mosaicity, low resolution),
and slightly higher for "good" data. Anyway, personally, I'm
doing several runs with different values of weighting, and check how it
affects the geometry (respective rmsd values).
Does it affect the result R value much?
Well yes, if you do distort like hell your protein side
chains & atoms, you will have everything that will fit into the electron
density, resulting in decreasing largely your stats, but you'll have little
biological significance (as an example: using a somehow too high value
of weighting, a lys was disrupted in 3 parts, to fit the blobs of density...).
Good luck.
Leo
==========================
Chavas Leonard M.G., Ph.D.
Structural Research Center
KEK,PF. 1-1 Oho
Tuskuba, Ibaraki - Japan
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PHS: +81(0)29-864-5200 (ext: 2682)
e-mail: [log in to unmask] URL: http://pfweis.kek.jp/~leo ==========================