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Hi I would appreciate few things for my protein structure. Data is 1.5A. This protein is of 400 residues. 2 molecule in the asymm unit. Missing residues are : N-terminal 8 residues, C-terminal 5 residues and in the middle residues 87-93. Final R(working) = 21% after refmac refinement. Some (not all) waters created into the structure. I am taking this structure into Shelxl for further refinement and building the missing residues. (1) During Shexl refinement how the missing residues I can fix. what kind of keyword I should use to fix the missing residues with a reasonable Occunany and B value. (2) In COOT how can I build acetate ions in place of waters. Or any other way I can call acetate ions. Thanks Sam _________________________________________________________________ Win a Zune™—make MSN® your homepage for your chance to win! http://homepage.msn.com/zune?icid=hmetagline