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Thank you for all the replies. @ Kris It is not the problem of single gas bubbles, but of solved gas. I prepared the sample in the middle of December and it still degases that means bubbles appear. They can be removed but after a few days new ones are coming up. I'm doing NMR and the shimming always gets worse if this appears. So it is difficult to get a good 3D dataset if in the middle of the measurement the sensitivity breaks down. I'm searching for a way to simple degas the sample. I put it in normal vacuum in tube, but that doesn't work good because of the small surface. Enlarging the surface would create the risk of abstracting too much water so that the concentration rises to fast and the Protein aggregates. Is there another way? Tomorrow I will try there syringe method. Justin