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I would be very much afraid that the only way to get the masks properly would be to edit them interactively. I used to do that in the PHASES package by Bill Furey and MapViewX which is still alive and kicking under the BnP flagship. I am pretty sure though that Dr. DVD will be online soon and will point out the correct succession of MAMA/RAVE/O commands ;-) A. On Feb 22, 2007, at 1:08, Jianghai Zhu wrote: > Forgot to mention that the NCSs are improper. > > > > On Feb 21, 2007, at 6:22 PM, Jianghai Zhu wrote: > >> Dear all, >> >> I have two low resolution (3.8 A) MAD data sets from two different >> space groups. There are 4 copies of my molecule in one space >> group and 2 copies in the other. The density-modified maps from >> these data sets are poor, but still allow me to build a crude >> model on them. All the domains should be at the right or close to >> right orientations and positions. I would like to try multi- >> crystal averaging to improve my maps. Since I already have the >> model, I used NCSMASK to make 6 masks for the 6 copies of my >> model. Then I used DMMULTI to perform the multi-crystal >> averaging. The maps came out of DMMULTI showed great >> improvement. Some densities were not seen before showed up >> nicely. However, after I read the manual carefully, I realize >> that DMMULTI would not take care of the mask overlap. So some >> regions in my density map between the NCS-related and symmetry- >> related molecules must be messed up. Could any expert out there >> give me some suggestions about how to make proper NCS masks for >> DMMULTI? Thanks at advance. >> >> Jianghai >