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Hi all, I'm looking for some advices on some "general hints" on how to carry out a MAD/SAD data collection. We had SeMet crystals that diffracted to ca. 3.5 Angs, with anomalous signal only at ca. 5-5.5 Angs, with diffraction decaying on brilliant beamlines (ID29 or ID23 at the ESRF) in a matter of ca. 300 degrees... Needless to say, that was not sufficient to solve the structure... We do have improved the crystals that look now nicer and bigger, and have some beamtime next weeks both at BM16 and ID29 at the ESRF. Assumed that we do see diffraction higher that 3 Angs, what would people suggest? Collecting first at the high energy remote for a SAD experiment and then going for peak and inflection point, or rather going for the peak first, then remote and ip? I personally would avoid the continuous switch of wavelengths, but I know there are some fans of this technique, either... Let the gurus talk! Any advice is obviously highly appreciated! Thanks in advance, Sebastiano and Claudio -- Sebastiano Pasqualato, PhD IFOM Istituto FIRC di Oncologia Molecolare via Adamello, 16 20139 Milano Italy tel +39 02 574 303 325 fax +39 02 574 303 310