Hi Tiancen,

nobody so far has suggested to mount the smallest crystals, 0.4x0.4x0.3

seems to be pretty large, this also means that the freezing procedure is

longer. Then the other question is do you freeze in the Cryostream or by

plunging into LN2, I'd prefer the latter technique.

Good luck,

Juergen

-- 

Jürgen Bosch

University of Washington

Dept. of Biochemistry, K-426

1705 NE Pacific Street

Seattle, WA 98195

Box 357742

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FAX: +1-206-685-7002