 |
 |
Hi, You might want to look at Berejnov, V., Husseini, N. S., Alsaiedc, O. A. & Thorne, R. E. (2006). J. Appl. Cryst. 39, 244-251. Regards, John John Rose Ph.D. Associate Professor B204B, The Fred C. Davison Life Sciences Complex 120 Green Street Department of Biochemistry and Molecular Biology University of Georgia Athens, GA 30602-7229 ========================================= Phone: 706-542-1750 Fax: 706-542-3077 On Jan 24, 2007, at 3:29 PM, Juergen Bosch wrote: > Hi Tiancen, > > nobody so far has suggested to mount the smallest crystals, > 0.4x0.4x0.3 > seems to be pretty large, this also means that the freezing > procedure is > longer. Then the other question is do you freeze in the Cryostream > or by > plunging into LN2, I'd prefer the latter technique. > > Good luck, > > Juergen > > > > -- > Jürgen Bosch > University of Washington > Dept. of Biochemistry, K-426 > 1705 NE Pacific Street > Seattle, WA 98195 > Box 357742 > Phone: +1-206-616-4510 > FAX: +1-206-685-7002