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Dear Developers, I have substantially increased the tolerance levels on spectra which contain J and residual dipolar couplings. That way I am still able to assign the peaks. However, sometimes this still doesn't seem to work. E.g. I have a peak at 119.370 ppm and the resonance I want to assign it to is at 120.557ppm, yet even when the tolerance is set to 2ppm in 15N for that spectrum the correct resonance does not appear as an option in the Assignment box (though there are other options which go up to a delta value of 1.75). There are other residues for which I have the same problem. Selecting 'Double tolerances' does not appear to change the options available when the tolerance is set to 2ppm (but it does at lower values). Is there a maximum number of residues which can be displayed in the assignment box? Is there a way I can force an assignment which I want to make? Is there a reason why it might be leaving out the assignment option which I want to make? Thanks. Vicky -- **************************************************** Dr. Victoria A. Higman Leibniz-Institut fuer Molekulare Pharmakologie (FMP) Robert-Roessle-Str. 10 13125 Berlin Germany Phone: +49-30-94793 223 E-mail: [log in to unmask] ****************************************************