Dear Tunicata Members We are trying to homogenise several ascidians to measure their metal content and also extract total genomic DNA. The samples we have are freshly frozen at -20degC and we tried mortar and pestle, hand-held electric homogeniser, and pulsifier techniques. None of these work terribly well because the tunic will not easily homogenise. Any advice/experience with this matter would be appreciated. Cheers Marcel Professor Marcel Jaspars Marine Natural Products Laboratory Department of Chemistry University of Aberdeen Old Aberdeen, AB24 3UE Scotland, UK Phone: +44 1224 272 895 Fax: +44 1224 272 921 Email: [log in to unmask] www: http://www.abdn.ac.uk/chemistry/research/mj/mj.hti