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Hi, When use the sections' overlay and then try to define VOIs several days after completing the analysis process, I get a beep and matlab reports an error. Here is a "citation": ??? Cant open image file. Error in ==> C:\spm2\spm_sample_vol.dll Error in ==> C:\spm2\spm_get_data.m On line 30 ==> Y(i,:) = spm_sample_vol(V(i),XYZ(1,:),XYZ(2,:),XYZ(3,:),0); Error in ==> C:\spm2\spm_regions.m On line 161 ==> y = spm_get_data(SPM.xY.VY,xSPM.XYZ(:,Q)); ??? Error while evaluating uicontrol Callback. The strange thing is that when I do the same process on my home computer it works just fine. Maybe it has something to do with the fact that this is the computer on which on the analysis was performed? Thanks for your answers, Avital