Dear all, Alex Hammers has pointed out to me that the tissue probability maps (in the tpm directory) released with SPM5b could be in better registration with the data of SPM2. This is just to warn you that a slightly different set of tissue probability maps will be released in a forthcoming update to SPM5b. The same original data will be used - but it will simply be better registered with the older data. If you plan to continue a study with the current set, then please make a backup copy of them, as downloading the updates is likely to over-write the current versions. The data in the tpm directory is used by the Segment button of SPM5b, so if you plan to continue using renamed versions of the current tissue probability maps, then you will need to change the Spatial->Segment->Custom->Tissue probability maps setting in order to use them. This can also be done by changing spm_defaults.m at around line 24, to something like: defaults.preproc.tpm = char(... fullfile(spm('Dir'),'tpm','old_grey.nii'),... fullfile(spm('Dir'),'tpm','old_white.nii'),... fullfile(spm('Dir'),'tpm','old_csf.nii')); % Prior probability maps Best regards, -John