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Dear SPM users, I’m a graduate student engaged in imaging studies. I have some SPECT data for some subjects in the activated condition and also in the rest (not activated) condition. I could compare the SPECT in the activated condition for multiple subjects and the SPECT in the rest condition for multiple subjects using ‘’multiple subjects condition and covariates’’. I want to compare the ‘’activated condition minus rest condition’’ in 1st group of subjects and 2nd group of subjects. ( the selection of subjects to the group 1 and 2 is irrelevant of the result of the SPECT. )I tried to do it in one-way ANOVA, for example, activated<sub1>- rest<sub1>-(activated<sub2>-rest<sub2>) for multiple subjects. My question is, Is this the proper way to do this kind of analysis? ( because the condition is determined in the acquisition of SPECT, and the subject selection between group 1 and group2 is an independent factor from the condition selection. ) If there’s a proper way to do this kind of analysis, could you please teach me how to do it. Thanks a lot for your kind help. Yours sincerely, Kazumi Iseki.