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Dear SPM lists, I have been working on VBM in order to get each hemispheric (rh/lh) GMV and have come to know that there are two strategies using getSegmentVolumes. The first strategy is to mask the all preprocessed images using masking (rh/lh) images, followed by calculating hemispheric GMV using getSegmentVolumes. The second strategy (according to an expert's suggestion) is to mask the all original images before preprocessing using masking (rh/lh) images, followed by VBM preprocessing to get final results. Which is more accurate? I myself am torn since I don't know the answer. Under my impression, however, it looks like the simple masking method (the first strategy) worked since a strong positive correlations existed between each hemispheric GMV (rh/lh) calculated by VBM and other brain imaging tool. Any suggestion or comments regarding this matter would be highly appreciated. Best, Akemi Developmental Biopsychiatry Research Program McLean Hospital, Belmont MA