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Did the images have .mat files to begin with? If so, then this would have caused problems. Step (4) is so that the originals revert back to their original orientation - which only works if the images don't already have .mat files. If they do have, then these should probably have been copied somewhere, and then copied back afterwards. Best regards, -John > I got the error message that the images do not all have the same > orientation and voxel size when I try to compare the nomalized images and > their fliped images even after I follow John Ashburner's instruction: > > 1) Make a copy of one of your spatially normalised images. > 2) Use the Display button to flip all the other images: > a) Set x zoom to -1 > b) Click the Reorient Images button, and select these images. > 3) Reslice all your flipped spatially normalised images to match > the saved unflipped image, using either the Coregister or Realign buttons > (reslice only). 4) Delete the .mat files created by Display. > > > I think there should be some problem that was ignored, but what's that? > I used Realign button in step 3 because when I used Coregister button, I > could only reslice 1 subject at one time and when number of subject is more > than one, it could not work.