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Hi, has anyone tried using spm_write_vol in spm5b? I am trying to use the suggestions from previous replies for writing my matlab matrix (128x128x64, imported as dicom and manipulated in matlab) to *.img format and I keep recieving error messages. However if I change this code: spm_defaults Volume = zeros(30,30,30); [x, y, z] = size(Volume); Info.mat = [2 0 0 0; 0 2 0 0; 0 0 2 0; 0 0 0 1] Info.fname = 'myimage.img'; Info.dim = [x,y,z,2]; Info.type = 2; % http://www.fil.ion.ucl.ac.uk/spm/software/spm99/#AzeFmt spm_write_vol(Info,Volume) to this code: spm_defaults Volume = zeros(30,30,30); [x, y, z] = size(Volume); Info.mat=[2 0 0 0; 0 2 0 0; 0 0 2 0; 0 0 0 1] Info.fname = 'myimage2.img'; Info.dim = [x, y, z]; %remove the 2 from above Info.dt = [2, 0]; %add this spm_write_vol(Info,Volume) it works. My questions: 1) how do I calculate Info.mat for my data, or is what is written above okay for everyone? 2) does Info.dt(1) = data type and Info.dt(2) = big or little endian? 3) The image that is written out shows the head rotated by 90 degrees, the anterior-posterior of the head is shown in the left-right direction. How can I rotate it back to "normal". Thanks, Anita