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Hello, I am unable to find adequate information regarding using nifti file format in SPM2. I used FSL 3.2b to strip the skull from colin27 brain image and want to use that for input into SPM2 for segmentation and normalization. FSL outputs a .nii file which I converted to ANALYZE two-file format using MRIcro. When I input the stripped colin image(target) and the MNI152 stripped image(source), I get an error "There is not enough overlap in the images to obtain a solution." When I view the colin image, there is nothing in the viewer. Is there a way to resolve this file format problem? Perhaps I need to use an older version of FSL? Or is there a better way to convert the *.nii to *.hdr/*.img format that SPM2 will read correctly? Thanks in advance for your help with this issue. Veronica