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I can
confirm that the Roche Albumine BCG and Sebia amido black method have the same
discrepancy. The regression equation according to Passing and Bablok
is:
Sebia
ALB amido black =1,384 * Roche ALB BCG - 10.7. So the
concentrations in the normal range are higher with Sebia than with Roche, though
our regression line is tilted in comparison with yours, giving progressively
higher values on higher concentrations.
Of
course it is known that different analytical assays measure different aspects of
molecules. Different dyes measure different molecular markers. That is even so
with differente amido black formulations!
Kind
regards,
Dr. Dirk
Bakkeren
Clinical chemist
Clinical laboratory
Dear colleagues,
Could I get some feedback on what you think of
this comparison of albumins on the Sebia electrophoretic system vs. albumins
on the Beckman Coulter LX20Pro.
There appears to be a large steady positive for
the Sebia method.
Many thanks,
Sean Maguire
Mater Hospital
Dublin 7
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