Dear SPM users. I was wondering if you could give me some ideas on how to accomplish the task that I have here. What we need is a ratio GM:WM:CSF from a specified voxel within a brain from a high resolution( about 1x1x1 mm) structural scan of a single subject. Do I have to normalize the brain prior to segmentation? I understand that I need to use prior images for segmentation, but, correct me if I am wrong, those prior images are just averages of lots of segmented brains? And without prior images it will just take longer to segment the brain? If I do need to normalize the brain before segmentation, where can I get the normalization matrix so that I could adjust the voxel position, size and shape also? Is it on the normalization output page under linear (affine) component? What about values contained in _sn.mat file? Thank you beforehand for your input! Best regards, Renat.