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Help! How far over the top should we go to give the best quality Lab service for these cans of worms? Pleural Fluids are expected to be be infected. Therefore, must the sample for pH be sent on ice if (instead of POCT) the sample takes a finite time to travel to the lab? Heparin mentioned: I don't see the quantity or type of heparin specified. Likewise, shouldn't a Lab assay for infected-fluid glucose use a fluoride tube sample? If Mycobacterium tuberculosis is a possibility, even a small possibility, must the handling & analysis be done in a suitable proper Safety Cabinet? Especially in these days of more resistant strains? Did they ask HSE? Thanks for the Thorax 2003 reference. Eyeballing the 4 papers quickly, I didn't spot any specifications of methodology or equipment for the glucose, protein, chol, TG, LD, amylase, or iso-amylase assays. The "packet inserts" in our reagent boxes do say which samples each assay is validated by the manufacturer for, presumably yours do too. Ours do NOT include pleural fluid for any of these analytes. Best wishes, Les > From: # David Ricketts <[log in to unmask]> > Reply-To: # David Ricketts <[log in to unmask]> > Date: Wed, 2 Jul 2003 11:26:48 +0100 > To: [log in to unmask] > Subject: Re: Pleural fluid pH > > Dear All > > We have a lot of TB in our area and are looking at safe practices for > handling TB+ve sample through blood gases. > Any suggestions welcome. > David Ricketts Lab Manager NMUH > > -----Original Message----- > From: Wahlstedt Juha [mailto:[log in to unmask]] > Sent: 02 July 2003 10:05 > To: [log in to unmask] > Subject: VS: Pleural fluid pH > > Hi Rachel > > We have been mesuring pleural fluid pH on blood gas analyser for severla > years. > Our protocol is: > - inject pleural fluid in heparin vacuum tube as soon as possilble. > - Sentrifuge the tube > - aspirate fluid from the tube in the syringe > - put a cloth catcher on a top of syringe (from Instrumentation > Laboratories) > - inject fluid to the analyzer > We havent got any broblems. We have been using IL BGE and Synthesis > analyzers. > > ________________________________ > Apulaisosastonhoitaja > MLT Juha Wahlstedt > > HUS > HYKS, Peijaksen sairaala > Kliininen laboratorio > Sairaalakatu 1 > 01400 VANTAA > > puh. 09 471 67252 > fax. 09 471 67571 > > e-mail (työ) mailto:[log in to unmask] > e-mail (koti) mailto:[log in to unmask] > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and clinical > community working in clinical biochemistry. > Please note, archived messages are public and can be viewed > via the internet. Views expressed are those of the individual and > they are responsible for all message content. > > ACB Web Site > http://www.acb.org.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ > > > CONFIDENTIALITY NOTICE > The contents of this e-mail are confidential and may also be privileged. If > you are not the addressee of this e-mail you may not copy, forward, disclose > or otherwise use it in any form whatsoever. If you have received this e-mail > in error please immediately telephone 020 8887 2345, and destroy and delete > the message from your computer. > NORTH MIDDLESEX HOSPITAL NHS TRUST > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and clinical > community working in clinical biochemistry. > Please note, archived messages are public and can be viewed > via the internet. Views expressed are those of the individual and > they are responsible for all message content. > > ACB Web Site > http://www.acb.org.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/